human osteosarcoma mg63 cell line  (ATCC)

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cytotoxicity effect on MG63 ( a ) and BMSCs ( b ) after 24 h of exposure to the tested samples’ extracts, evaluated via the release of LDH enzyme. Complete growth media was used as a negative control, and lysed cells were used as a positive control. Results are expressed as mean ± standard deviation (n ≥ 3, independent samples).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Negative Control, Positive Control, Standard Deviation

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cellular viability of MG63 ( a ) and BMSCs ( b ) cultured with 100% extracts from each sample, for 1 day. Complete growth media was used as a control. The dashed line indicates the 70% cut-off for a toxic effect. Results are expressed as mean ± standard deviation (n ≥ 3, independent samples, * p < 0.05).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Cell Culture, Control, Standard Deviation

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cellular viability of MG63 ( a ) and BMSCs ( b ) cultured with 100% extracts from each sample for 3 days. Results are expressed as mean ± standard deviation (n ≥ 3; independent samples; * p < 0.05).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Cell Culture, Standard Deviation

Journal: Molecular Nutrition & Food Research

Article Title: Saucerneol Inhibits the Growth, Migration, and Invasion of Osteosarcoma Cells In Vitro and Prevents Metastasis‐Associated Osteolysis Ex Vivo

doi: 10.1002/mnfr.70187

Figure Lengend Snippet: Effects of Sauc on cell death, apoptotic cell morphology, and migration in human osteosarcoma cells. (A, B) MG63 cells (A) and SJSA‐1 cells (B) were seeded in 96‐well plates and cultured with Sauc at the indicated concentrations for 24 h. The numerical values of cytotoxicity are presented as a bar graph normalized to those of the control. (C, D) MG63 cells (C) and SJSA‐1 cells (D) were seeded in six‐well plates and cultured with Sauc at the indicated concentrations for 24 h. The morphological changes were observed using the Olympus CKX53 inverted microscope. Scale bar: 200 µm. (E, F) After 24 h of Sauc treatment of MG63 cells (E) and SJSA‐1 cells (F), cell migration was assessed using the wound healing assay. The migration rate is presented as a bar graph. Scale bar: 200 µm. All values are expressed as mean ± SD of the results from three independent experiments. * Indicates a statistically significant difference, with p < 0.05 compared to the control.

Article Snippet: Human osteosarcoma cell lines MG63 (#CRL‐1427) and SJSA‐1 (#CRL‐2098) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Cell Culture, Control, Inverted Microscopy, Wound Healing Assay

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Partitioning of different cell types within the various ATPS scaffold formulations. Brightfield and confocal (merged) images representative of cells labelled with DAPI to show the nucleus (blue) of the different cell types (A549, C2C12, MG63, HBMSCs), and GelMA marked with rhodamine B to show the inner phase (red) of the scaffolds at 0 - 9 - 36 g/L. In all cell types, images were taken both under conditions where GelMA was not chemically cross-linked (-GelMA) and under conditions where GelMA was chemically cross-linked (+GelMA). Scale bars: (a, b, c, d) 100 μm. Mean ± S.D. n=3.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques: